Journal: Nature protocols

Article Title: Use of human induced pluripotent stem cell-derived cardiomyocytes to assess drug cardiotoxicity

doi: 10.1038/s41596-018-0076-8

Figure Lengend Snippet: Troubleshooting table

Article Snippet: Matrigel (human embryonic stem cell (hESC)-qualified; BD Sciences, cat. no. 354277) PBS (Life Technologies, cat. no. 20012050) DMEM/F12 medium (Thermo Fisher Scientific, cat. no. 11320033) RPMI 1640 medium (Thermo Fisher Scientific, cat. no. 11835055) Glucose-free RPMI 1640 medium (Thermo Fisher Scientific, cat. no. 11879–020) L-lactic acid (Wako Chemicals, cat. no. 129–02666) E8 medium (Thermo Fisher Scientific, cat. no. A1517001) L-ascorbic acid 2-phosphate (Wako Chemicals, cat. no. 321–44823) Sterile water for injection (WFI) (Corning, cat. no. 25–055-CV) Tyrode’s solution (Sigma, cat. no. T2397) Recombinant human albumin (ScienCell, cat. no. OsrHSA-100) CHIR99021 (Thermo Fisher Scientific, cat. no. 508306) Wnt-C59 (Biorbyt, cat. no. orb181132) Rho kinase (ROCK) inhibitor (Tocris, cat. no. 1254) 0.5 M EDTA (Thermo Fisher Scientific, cat. no. 15575020) DMSO (Sigma-Aldrich, cat. no. D-2650) TrypLE Express (Thermo Fisher Scientific, cat. no. 12605010) FBS (Thermo Fisher Scientific, cat. no. 10438026) PrestoBlue cell viability reagent (Thermo Fisher Scientific, cat. no. A13261) Wheat germ agglutinin–Alexa Fluor 488 conjugate (Thermo Fisher Scientific, cat. no. {"type":"entrez-nucleotide","attrs":{"text":"W11261","term_id":"1285566","term_text":"W11261"}} W11261 ) Calcein-AM (Thermo Fisher Scientific, cat. no. C3100MP) Trypan blue dye (Thermo Fisher, cat. no. 15250061)

Techniques: Produced, Selection, Metabolic Labelling, Concentration Assay, Fluorescence, Imaging